John Finer

John Finer
John Finer
Professor
Office: 
214 Williams Hall
Wooster
Phone: 
(330) 263-3880
Areas Of Interest: 
Genetic Transformation and Transgene
Expression in Crop Plants

AREA OF EXPERTISE:

Research efforts in the laboratory have focused somatic embryogenesis, genetic transformation and promoter analysis in crop plants. Although the main emphasis in my laboratory is currently on soybean, we have additional experience with transformation of wheat, maize, sunflower, tomato, bentgrass, cotton, white pine, Chinese yam, Christmas cactus and Ohio buckeye. Embryogenic tissues are used for both basic studies on plant growth and development and as targets for transformation work. For our promoter work, we use image analysis to quantify expression of the gfp gene, under regulatory control of different promoters. Automated image collections is facilitated using a custom-designed robotics system, coupled with high-throughput image analysis.

WEBSITE

http://www.oardc.ohio-state.edu/plantranslab/default.htm

Works

Chapters

Finer JJ, "Visualizing transgene expression." In Plant Transformation Technologies. Edited by CN Stewart, A Touraev, V Citovsky, T Tzfira. Hoboken: Wiley Publishing Co. 2011.
Finer JJ, "Plant Nuclear Transformation" In Genetic Modification of Plants, Biotechnology in Agriculture and Forestry. Edited by Springer. Dordrecht: Springer. 2010.

Conferences

Hu,Bizhen; Scheerens,Joseph,C; Cardina,John; Finer,John,J, "Metabolite Production in Plant Cell Cultures of Burdock (Arctium sp.)" 2012.
Hernandez-Garcia,Carlos,M; Nemes,Cheri; Jones,Michelle,L; Rushton,Paul,J; Finer,John,J, "Wound-induction of GmERF Promoters in Soybean" Society for In Vitro Biology 2012.
Zhang,Zhifen; Finer,John,J, "Development of a Transformation and Regeneration System for Sunflower (Helianthus annus)" 2011.
Hernandez-Garcia,Carlos,M; Nemes,Cheri; De la Torre,Carola; Semsang,Nuananong; Jones,Michelle,L; Finer,John,J, "GmERF Promoters Give High Wound-inducible Expression in Transgenic Soybean" Society for In Vitro Biology 2011.
De La Torre,Carola,M; Hernandez-Garcia,Carlos,M; Bouchard,Robert,A; Finer,John,J, "Dissection of the Strong Soybean Ubiquitin Promoter (Gmubi) Reveals Essential Regions Responsible for Regulating High Levels of Gene Expression" 2011.
Waterland, NL, Finer, JJ, Jones, ML, "Abscisic Acid Applications Decrease Stomatal Conductance and Delay Wilting in Drought-stressed Chrysanthemums" 106th Annual Conference of the American-Society-for-Horticultural-Science 2010.
Bouchard,R,A; Chiera,J,M; Finer,J,J, "Isolation and characterization of a soybean (Glycine max) polyubiquitin (Gmubi) promoter." 2007.
Chiera,J,M; Finer,J,J, "Evaluation of an automated image analysis system for factors which stabilize gene expression." Society for In Vitro Biology 2006.
Chiera,J,M; Streeter,J,G; Nemes,C,A; Finer,J,J, "The effect of Myo-inositol concentration on pinitol levels in Inositol methyl transferase (imt)-containing transgenic embryonic soybean" Society for In Vitro Biology 2005.

Journal Articles

King JL, Finer JJ, McHale LK, "Development and optimization of agroinfiltration for soybean" Plant Cell Reports 34 (2015) 133-140
DeLaTorre CM, Finer JJ, "The intron and 5’ distal region of the soybean Gmubi promoter contribute to very high levels of gene expression in transiently and stably transformed tissues" Plant Cell Reports 34 (2015) 111-120
Hernandez-Garcia CM, Finer JJ, "Identification and validation of promoters and cis-acting regulatory elements" Plant Science 217–218 (2014)
Lin J, Guo J, Finer J, Dorrance A, Redinbaugh M, Qu F, "The bean pod mottle virus RNA2-encoded 58K protein is required in cis for RNA2 accumulation" Journal of Virology 88 (2014) 3213-3222
Lin,Junyan; Ali,Ahmed,Khamis; Chen,Ping; Ghabrial,Said; Finer,John; Dorrance,Anne; Redinbaugh,Peg; Qu,Feng, "A stem-loop structure in the 5 ' untranslated region of bean pod mottle virus RNA2 is specifically required for RNA2 accumulation" JOURNAL OF GENERAL VIROLOGY 94 (2013) 1415-1420
Rushton,Deena,L; Tripathi,Prateek; Rabara,Roel,C; Lin,Jun; Ringler,Patricia; Boken,Ashley,K; Langum,Tanner,J; Smidt,Lucas; Boomsma,Darius,D; Emme,Nicholas,J; Chen,Xianfeng; Finer,John,J; Shen,Qingxi,J; Rushton,Paul,J, "WRKY transcription factors: key components in abscisic acid signalling" PLANT BIOTECHNOLOGY JOURNAL 10 1 (2012) 2-11
Pomeranz,Marcelo; Finer,John; Jang,Jyan-Chyun, "Putative molecular mechanisms underlying tandem CCCH zinc finger protein mediated plant growth, stress, and gene expression responses." Plant signaling & behavior 6 5 (2011) 647-651
Pomeranz,Marcelo; Zhang,Li; Finer,John; Jang,Jyan-Chyun, "Can AtTZF1 act as a transcriptional activator or repressor in plants?" Plant signaling & behavior 6 5 (2011) 719-722
Hernandez-Garcia,Carlos,M; Bouchard,Robert,A; Rushton,Paul,J; Jones,Michelle,L; Chen,Xianfeng; Timko,Michael,P; Finer,John,J, "High level transgenic expression of soybean (Glycine max) GmERF and Gmubi gene promoters isolated by a novel promoter analysis pipeline" BMC PLANT BIOLOGY 10 (2010)
Waterland,Nicole,L; Finer,John,J; Jones,Michelle,L, "Benzyladenine and Gibberellic Acid Application Prevents Abscisic Acid-induced Leaf Chlorosis in Pansy and Viola" HORTSCIENCE 45 6 (2010) 925-933

Patents

Finer JJ, Benzle KA, Finer KR, Taylor CG. Agrobacterium Strains for Plant Transformation and Related Materials and Methods. Filed Apr 2014
John J. Finer and Robert A. Bouchard. Highly Active Soybean Promoters and Uses Thereof. Filed Jan 2013
John J Finer; Robert A Bouchard. Highly Active Soybean Promoters and Uses Thereof. 8395021 Filed Apr 2008 and Issued Apr 2013.
John J Finer. Condensation reducing/eliminating container. Filed Jun 2006
Finer,John,J; Trick,Harold,N. Method for transforming plant tissue by sonication. 5693512 Filed Feb 1996 and Issued Nov 1997.

Presentations

"Isolation and evaluation of novel Agrobacterium strains for transformation of soybean and sunflower." InCrown Gall Meetings. West Lafayette: 2014.
"Improvements in sunflower transformation using cotyledon explants from mature seeds." InCrown Gall Meeting. West Lafayette: 2014.
"Identification and validation of regulatory elements from soybean promoters." InPurdue University Department of Agronomy Seminar Series. 2014.
"Soybean Promoteromics." InThe Ohio State University Department of Plant Pathology Seminar Speaker. 2014.
"Isolation and Characterization of Soybean Urease Promoters." InSoy2014. Minneapolis: 2014.
"A leading intron of a soybean elongation factor 1A gene interacts with soybean promoter elements to regulate gene expression." InSociety for In Vitro Biology Annual Meeting. Savannah: 2014.
"Isolation and evaluation of new Agrobacterium strains for transformation of soybean and sunflower." InSociety for In Vitro Biology Annual Meeting. Savannah: 2014.
"Publication Ethics, Plagiarism." InSociety for In Vitro Biology Annual Meeting. Savannah: 2014.
"Development of Sunflower Transformation through the Use of Cotyledon Explants from Mature Seeds." InSociety for In Vitro Biology Annual Meeting. Savannah: 2014.
"Transgene expression analysis in soybean." InOhio State University Department of Horticulture and Crop Science Seminar Series. 2014.