Research efforts in the laboratory have focused somatic embryogenesis, genetic transformation and promoter analysis in crop plants. Although the main emphasis in my laboratory is currently on soybean, we have additional experience with transformation of wheat, maize, sunflower, tomato, bentgrass, cotton, white pine, Chinese yam, Christmas cactus and Ohio buckeye. Embryogenic tissues are used for both basic studies on plant growth and development and as targets for transformation work. For our promoter work, we use image analysis to quantify expression of the gfp gene, under regulatory control of different promoters. Automated image collections is facilitated using a custom-designed robotics system, coupled with high-throughput image analysis.
The five (5) most recent works for each category are listed below. View all works.
Finer JJ. "Visualizing transgene expression.." In Plant Transformation Technologies. Edited by CN Stewart, A Touraev, V Citovsky, T Tzfira. Hoboken, NJ: Wiley Publishing Co, January 2011.
Hu, Bizhen, Scheerens,Joseph, Cardina, John, Finer, John. "Metabolite Production in Plant Cell Cultures of Burdock (Arctium sp.)." (July 2012)
Hernandez-Garcia,Carlos,M; Nemes,Cheri; Jones,Michelle,L; Rushton,Paul,J; Finer,John,J. "Wound-induction of GmERF Promoters in Soybean." Society for In Vitro Biology. (July 2012)
Zhang,Zhifen; Finer,John,J. "Development of a Transformation and Regeneration System for Sunflower (Helianthus annus)." (June 2011)
Hernandez-Garcia,Carlos,M; Nemes,Cheri; De la Torre,Carola; Semsang,Nuananong; Jones,Michelle,L; Finer,John,J. "GmERF Promoters Give High Wound-inducible Expression in Transgenic Soybean." Society for In Vitro Biology. (June 2011)
De La Torre,Carola,M; Hernandez-Garcia,Carlos,M; Bouchard,Robert,A; Finer,John,J. "Dissection of the Strong Soybean Ubiquitin Promoter (Gmubi) Reveals Essential Regions Responsible for Regulating High Levels of Gene Expression." (June 2011)
Lin,Junyan; Ali,Ahmed,Khamis; Chen,Ping; Ghabrial,Said; Finer,John; Dorrance,Anne; Redinbaugh,Peg; Qu,Feng. "A stem-loop structure in the 5 ' untranslated region of bean pod mottle virus RNA2 is specifically required for RNA2 accumulation."; JOURNAL OF GENERAL VIROLOGY.. Vol. 94 (June 2013): 1415-1420.