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Dietz Bauer
Professor Emeritus
Department of Horticulture and Crop Science
Email: bauer.7@osu.edu
at OSU since Emeritus Since October 2005
AREA OF EXPERTISE:
Role of bacterial quorum sensing in rhizosphere biology.
1) We discovered that various higher plants secrete compounds that mimic the N-acyl homoserine (AHL) signals used by many bacteria for quorum sensing (Teplitski et al, Mol. Plant-Microbe Interact. 13:637. 2000). These compounds are potentially useful to the plant in disrupting or manipulating quorum sensing regulation in bacteria cells trying to infect the host. We are currently working on chemical identification of the plant mimic compounds.
2) We recently discovered that Medicago truncatula, a model plant closely related to alfalfa, responds very extensively and specifically to nanomolar concentrations of bacterial AHL quorum sensing signals (Mathesius et al, PNAS 100: 1444. 2003), indicating that higher plants can detect subthreshold levels of the AHL signals used by bacteria during infection and can use this information to make diverse responses to the presence of such bacteria. We are currently testing the ability of the model nematode, C. elegans, to detect and respond to bacterial AHLs and characterizing the effects of AHL treatment on plant responses to bacteria.
3) We have recently identified over 100 proteins in Sinorhizobium meliloti, the nitrogen-fixing bacterial symbiont of M. truncatula, that are responsive to added AHL signals from the bacteria. The AHLs synthesized by two different strains of S. meliloti have been chemically identified. We are currently working on the identification of genes and proteins regulated by specific AHL signals and receptors in S. meliloti.
4) We have recently detected at least 15-20 different AHL signal-mimic compounds produced by M. truncatula and have shown that AHL-regulated genes in S. meliloti are affected by addition of purified AHL mimic compounds from the unicellular green alga Chalmydomonas.
Date of Birth: July 29, 1939. Place of Birth: Ann Arbor, Michigan.
Education:
B.S. Utah State University (Chemistry), 1961.
Ph.D. University of Colorado (Biochemistry), 1971.
Professional Experience:
1963-1965 University of the Philippines, Peace Corps Volunteer.
1965-1966Teaching Assistant, University of Colorado.
1969-1971 Research Assistant, University of Colorado.
1971-1973 Postdoctoral Fellow, Michigan State University.
1973-1974 Postdoctoral Research Associate, Cornell University.
1974-1980 Staff Scientist, C.F. Kettering Research Laboratory.
1980-1984 Senior Investigator, C.F. Kettering Research Lab
1984-1985 Research Leader, Battelle-Kettering Research Lab
1986- 1999 Associate Professor, Ohio State University.
1999- 2005 Professor, Horticulture & Crop Science, OSU
2005 - Present Professor Emeritus, Horticulture & Crop Science, OSU
Professional Societies:
American Society for Microbiology; American Society Plant Physiologists;
International Society for Molecular Plant-Microbe Interactions
Professional Services:
Editorial Board, Plant Physiology, 1977-82, 1988-92
Editorial Board, Symbiosis, 1985--
Editorial Board, Planta, 1986-94
USDA Competitive Grants Program:
Review Panel Member: Stress Program, 1979-80
Review Panel Member: Nitrogen Fixation Program, 1983-84
Review Panel Member: Stress Program, 1986
Program Manager, Nitrogen Fixation Program, 1988
Technical Consultant, National Workshop on Nitrogen Fixation and Crop
Productivity, Taichung, Taiwan, 1981
Technical Consultant, Agrigenetics Research Crop, 1982-85
Technical Consultant, U.S. National Research Council, Conference on
Biotechnology in Agriculture, Jakarta, Indonesia, 1986
Technical Consultant, Biotechnica Corp, 1989, 1990
Technical Consultant, U.S. National Academy of Science, preparation of
Congressional report on biological control in agriculture, 1989
Technical Consultant, development of safety guidelines for large scale release of
genetically modified biofertilizer microorganisms for OECD, Ottawa, 1992,
Paris, 1993.
Proposal and Site Reviewer for National Science Foudation, National Science
and Technology Centers Program, 1991-1993
Technical Consultant, US Environmental Protection Agency Workshop on
testing risks associated with release of GEMs, Washington DC, 1994.
Recognitions and Awards:
Certificate of Merit, National Merit Scholarships, 1957.
Co-author, basic process patent, thermal diazotype reproduction, 1960.
Dupont Predoctoral Teaching Fellowship, 1966.
National Institutes of Health Predoctoral Fellowship, 1967-69.
National Institutes of Health Postdoctoral Fellowship, 1971.
AEC Plant Research Laboratory Postdoctoral Fellowship, 1972.
George Olmstead Award from the American Paper Institute, 1973.
Research Fellowship from Organization for Economic and Cooperative
Development, 1994
National Science Foundation Grants. Principal Investigator.
The Role of Lectins in Plant-Microorganism Interactions, 1975-1983 Symbiotic
Infection of Soybean by Bradyrhizobium japonicum, 1983-86
Chemotaxis in Root Colonization and Symbiotic Infection, 1989-93
USDA Competitive Grant Program Awards. Principal Investigator.
Regulation of Lectin Receptor Synthesis byB. japonicum, 1978-80.
Mechanisms by which Soybean Plants Optimize Infection and Nodulation by
Rhizobia, 1980-1989
Feedback Regulation of Nodulation, 1990-91
Root Mucilage 1995-98
Quorum sensing in S. meliloti and the role of signal mimics fromthe host plant. 2001-2003
DOE Basic Energy Science Grants. Principal Investigator. The Basis for
Competitiveness of Bradyrhizobium japonicum Strains in Nodulation of
Soybean, 1981-1989.
Agrigenetics Corporation Research Grant.
Selection of Competitive Bradyrhizobium japonicum Strains, 1982-86.
Publications:
Bauer, W. D., Talmadge, K. W., Keegstra, K. and Albersheim, P.
1973. The structure of plant cell walls II. The hemicellulose of the walls of
suspension-cultured sycamore cells. Plant Physiol. 51:174-187.
Keegstra, K., Talmadge, K. W., Bauer, W. D. and Albersheim, P.
1973. The structure of plant cell walls III. A model of the walls of
suspension-cultured sycamore cells based on the interconnections of the
macromolecular components. Plant Physiol. 51:188-196.
Talmadge, K. W., Keegstra, K., Bauer, W. D. and Albersheim, P.
1973. The structure of plant cell walls I. The macromolecular components of
the walls of suspension-cultured sycamore cells with a detailed analysis of the
pectic polysaccharides. Plant Physiol. 51:158-173.
Albersheim, P., Bauer, W. D., Keegstra, K. and Talmadge, K. W.
1973. The structure of the wall of suspension-cultured cells. In Biogenesis of
Plant Cell Wall Polysaccharides. F. Loewus, Ed., Academic Press, New York,
pp. 117-148.
Bauer, W. D., Bateman, D. F. and Whalen, C. H. 1977. Purification of
an endo-1,4-galactanase produced by Sclerotinia sclerotiorum: Effects on
isolated plant cell walls and potato tissue. Phytopathology 67:862-868.
Bhuvaneswari, T. B., Pueppke, S. G. and Bauer, W. D. 1977. The role
of lectins in plant-microorganism interactions I. Binding of soybean lectin to
rhizobia. Plant Physiol. 60:486-491.
Bauer, W. D. 1977. Lectins as determinants of specificity in
legume-Rhizobium symbiosis. In Genetic Engineering for Nitrogen Fixation, A.
Hollaender, Ed., Plenum Press, New York, pp. 283-298.
Bauer, W. D., Bhuvaneswari, T. V. and Pueppke, S. G. 1977. The
involvement of soybean lectin in host plant recognition of the nitrogen-fixing
symbiont Bradyrhizobium japonicum. In Cell Wall Biochemistry Related to
Specificity in Host Plant Pathogen Interactions. B. Solheim, J. Raa, Eds.,
Universitets forlaget, Trms, pp. 377-380.
Bauer, W. D. 1978. Plant cell walls. Molecular and Cellular Plant Physiology,
H.E. Street, Ed., Chapter 1, Blackwell Scientific Publications, Ltd., Oxford.
Pueppke, S. G., Bauer, W. D., Keegstra, K. and Ferguson, A. 1978.
The role of lectins in plant-microorganism interactions II. Distribution of
soybean lectin in tissues of Glycine max Merr. Plant Physiol. 61:779-784.
Bhuvaneswari, T. V. and Bauer, W. D. 1978. The role of lectins in plant
microorganism interactions III. The influence of rhizosphere/rhizoplane culture
conditions on the soybean lectin-binding properties of rhizobia. Plant Physiol.
62:71 74.
Calvert, H. E., Lalonde, M., Bhuvaneswari, T. V. and Bauer, W. D.
1978. Role of lectins in plant-microorganism interactions IV. Ultrastructural
studies of the binding sites for soybean lectin on the cell surfaces of Rhizobium
japonicum. Can. J. Microbiol. 24:785-793.
Bauer, W. D. and Bhuvaneswari, T. V. 1979. The possible role of lectins
in legume/Rhizobium symbiosis and other plant-microorganism interactions. In
Recent Advances in Biological Nitrogen Fixation. N.S. Subba Rao, Ed., Holmes
& Meiers Publishers, New York, pp. 344-379.
Bhuvaneswari, T. V., Turgeon, B. G. and Bauer, W. D. 1980. Early
events in the infection of soybean (Glycine max L. Merr.) by Rhizobium
japonicum I. Localization of infectible root cells. Plant Physiol. 66:1027-1031.
Bauer, W. D. 1980. Role of soybean lectin in the soybean-Rhizobium
japonicum symbiosis. In Nitrogen Fixation, Vol. II, W. E. Newton and W. H.
Orme-Johnson, Eds., University Park Press, Baltimore, pp. 205-214.
Mort, A. J. and Bauer, W. D. 1980. Composition of the capsular and
extracellular polysaccharides of Rhizobium japonicum. Changes with culture
age and correlations with binding of soybean seed lectin to the bacteria. Plant
Physiol. 66:158-163.
Bauer, W. D. 1981. Infection of legumes by rhizobia. Ann. Rev. Plant
Physiol. 32:407-449.
Bhuvaneswari, T. V., Bhagwat, A. A. and Bauer, W. D. 1981.
Transient susceptibility of root cells in four common legumes by rhizobia. Plant
Physiol. 68:1144-1149.
Turgeon, B. G. and Bauer, W. D. 1982. Early events in the infection of
soybean by Rhizobium japonicum II. Time course and cytology of the initial
infection process. Can. J. Bot. 60:152-161.
Law, I. J., Yamamoto, Y., Mort, A. J. and Bauer, W. D. 1982.
Nodulation of soybean by Rhizobium japonicum mutants with altered capsule
synthesis. Planta 154:100-109.
Mort, A. J. and Bauer, W. D. 1982. Application of two new methods for
cleavage of polysaccharides into specific oligosaccharide fragments. Structure of
the capsular and extracellular polysaccharides of Rhizobium japonicum that
bind soybean lectin. J. Biol. Chem. 257:1870-1875.
Bauer, W. D. 1982. The initiation of infections in legumes by rhizobia. In
The Physiological and Biochemical Basis of Plant Infection. Y. Asada and C.
Vance, Eds., Japan Sci. Soc. Press, Tokyo, pp. 61-78.
Pierce, M. and W. D. Bauer. 1983. A rapid regulatory response governing
nodulation in soybean. Plant Physiol. 73:286-290.
Turgeon, B. G. and Bauer, W. D. 1983. Spot inoculation of soybean with
Rhizobium japonicum. Protoplasma 115:122-128.
Bhuvaneswari, T. V., Mills, K. K., Crist, D. K., Evans, W. R. and
Bauer, W. D. 1983. Effects of culture age on the symbiotic infectivity of
Rhizobium japonicum. J. Bacteriol. 153:443-451.
Calvert, H. E., Pence, M., Pierce, M., Malik, N. S. A. and Bauer,
W. D. 1984. Anatomical analysis of the development and distribution of
Rhizobium infections in soybean roots. Can. J. Bot. 62:2375-2384.
Crist, D. K., Mills, K. K., Bauer, W. D. and Evans, W. R. 1984.
Effect of long term incubation in water on the nodulation ability of Rhizobium
japonicum. Appl. Environ. Microbiol. 47:895-900.
Malik, N. S. A., Pence, M., Calvert, H. E. and Bauer, W. D. 1984.
Rhizobium japonicum infection and nodule development in soybean are affected
by exposure of the cotyledons to light. Plant Physiol. 75:90-94.
Turgeon, B. G. and Bauer, W. D. 1985. Ultrastructure of infection thread
development during the infection of soybean by Rhizobium japonicum. Planta
163:328-349.
Vesper, S. J. and Bauer, W. D. 1985. Studies on the attachment of rhizobia
to soybean roots. Symbiosis 1:139-162.
Mills, K. K. and Bauer, W. D. 1985. Attachment of rhizobia to clover
roots. J. Cell Sci. Suppl. 2:333-345.
Vesper, S. J. and Bauer, W. D. 1986. Role of pili in attachment of rhizobia
to soybean roots. Appl. Environ. Microbiol. 52:134-141.
Malik, N. S. A., Calvert, H. E., and Bauer, W. D. 1987. Nitrate
induced regulation of nodule formation in soybean. Plant Physiology.
84:266-271.
Vesper, S. J., N. S. A. Malik and W. D. Bauer. 1987. Transposon
mutants of Bradyrhizobium japonicum altered in attachment to host roots.
Appl. Environm. Microbiol. 53:1959-1961.
Bhuvaneswari, T. V., A. P. Lesniak and W. D. Bauer. 1988. Efficiency
of nodule initiation in cowpea and soybean. Plant Physiol. 86:1210-1215.
Caetano-Anolles, G., L. G. Wall, A. T. DeMicheli, E. M. Macchi,
W. D. Bauer and G. Favelukes. 1988. Motility and chemotaxis affect
nodulating efficiency of Rhizobium meliloti. Plant Physiol. 86:1228-1235.
Caetano-Anolles, G. and W. D. Bauer. 1988. Initiation of nodules on
alfalfa roots by nod gene mutants and co-inoculated wildtype Rhizobium
meliloti. Planta. 174:385-395.
Caetano-Anolles, G., D. K. Crist-Estes and W. D. Bauer. 1988.
Chemotaxis of Rhizobium meliolti to the plant flavone luteolin requires
functional nodulation genes. J. Bacteriol. 170:3164-3169.
Malik, N.S.A. and W.D. Bauer. 1988. When does the self-regulatory
response elicited in soybean root after inoculation occur? Plant Physiol.
88:537-539.
Caetano-Anolles, G. and W. D. Bauer. 1988. Feedback regulation of
nodulation in alfalfa. Planta 175:546-557.
Caetano-Anolles, G., G. Favelukes and W. D. Bauer. 1990.
Optimization of seed surface sterilization for legumes. Crop Science
30:708-712.
Caetano-Anolles, G. and W.D. Bauer. 1990. Rhizobium meliloti host
specificity mutants have additive effects in situ on initiation of alfalfa nodules.
Planta, 181:109 116
Caetano-Anolles, G. and W.D. Bauer. 1990. Rhizobium meliloti
exopolysaccharide mutants elicit feedback regulation of nodule formation in
alfalfa. Plant Physiology, 91:368-374
Bauer, W.D. and G. Caetano-Anolles. 1990. Chemotaxis, induced gene
expression and competitiveness in the rhizosphere. Plant and Soil. 129:45-52
Bauer, W.D. 1991. Motility and chemotaxis in the life of rhizobia. In: Plant
Biotechnology and Development. P.M. Gresshoff, ed. CRC Press, Ann Arbor.
pp 17-24
Dharmatilake, A.J. and W.D. Bauer. 1992. Chemotaxis of Rhizobium
meliloti towards nodulation gene inducing compounds from alfalfa roots. Appl.
Environm. Microbiol. 58:1153-1158
Caetano-Anolles, G., E. Wrobel-Boerner and W.D. Bauer. 1992.
Growth and movement of spot-inoculated Rhizobium meliloti on the root
surface of alfalfa. Plant Physiol. 98:1181-1189
Robinson, J.B., O.H. Tuovinen and W.D. Bauer. 1993. Role of divalent
cations in the subunit associations of complex flagella from Rhizobium meliloti.
J. Bacteriol. 174:3896-3902
Robinson, J.B. and W.D. Bauer. 1993. Chemotaxis of Rhizobium meliloti
towards C4-dicarboxylic acids and its relation to carboxylate transport
functions. J. Bacteriol. 175:2284-2291
Mathews, A., W.D. Bauer, J. Brockwell, B.J. Carroll, R. R. Gault
and P. M. Gresshoff. 1993. Rhizosphere colonization by Bradyrhizobium
japonicum is related to extent of nodulation of Glycine max cv. Bragg and its
supernodulating mutants. Soil Biol and Biochem. 25:613-617
Wei, X. and W.D. Bauer. 1998. Starvation-induced changes in motility,
chemotaxis and flagellation of Rhizobium meliloti. Appl. Environ. Microbiol.
64:1708 1714.
Wei, X. and W.D. Bauer. 1999. Tn5-induced and spontaneous switching of Sinorhizobium meliloti to enhanced swarming behavior. Appl. Environ. Microbiol. 65:1228-1235.
Han, D.Y., D.L. Coplin, W.D. Bauer and H.A.J. Hoitink. 2000. Induction of systemic resistance to bacterial leaf spot of radish by Pantoea agglomerans E278Ar from compost in a novel growth pouch bioassay. Phytopathology. 90: 327-332
Teplitski, M., J.B. Robinson and W.D. Bauer. 2000. Plants secrete substances that mimic bacterial N-acyl homoserine lactone signal activities and disrupt cell density dependent behaviors in associated bacteria. Mol. Plant-Microbe Interact. 13:637-648.
Knee, E. M., F.C Gong, M. Gao, M. Teplitski, A.R. Jones, A. Foxworthy, A.J. Mort and W.D. Bauer. 2001. Root mucilage from pea and its utilization by rhizosphere bacteria as a sole carbon source. Mol. Plant-Microbe Interact. 14:775-784.
Bauer, W.D. and M. Teplitski. 2001. Can plants manipulate bacterial quorum sensing? Austral. J. Plant Physiol. 28:913-921.
Pellock, B.J., M. Teplitski, W.D. Bauer, R.P. Boinay and G.C. Walker. 2002. A LuxR homolog controls production of symbiotically active EPS II by Sinorhizobium meliloti. J. Bacteriol. 184:5067-5076.
Bauer, W.D. and J.B. Robinson. 2002. Disruption of bacterial quorum sensing by other organisms. Current Opinon in Biotechnology 13:234-237
Mathesius, U., S. Mulders, M. Gao, M. Teplitski, G. Caetano-Anolles, B. G. Rolfe and W. D. Bauer. 2003. Extensive and specific responses of a eukaryote to bacterial quorum sensing signals. Proc. Nat. Acad. Sci. 100:1444-1449.
Rolfe, B.G., Mathesius U., Djordjevic, M., Weinman, .J, Hocart, C., Weiller G., and Bauer, W.D. 2003. Proteomic analysis of legume-microbe interactions. Comparative and Functional Genomics. 4: 225-228.
Chen, H. Teplitski, M., M. Gao, J. B. Robinson, B. G. Rolfe, and W. D. Bauer. 2003. Proteomic analysis of wildtype Sinorhizobium meliloti responses to N-acyl homoserine lactone quorum sensing signals. J. Bacteriol. 185:5029-5036.
Von Bodman, S.B., W.D. Bauer and D.L. Coplin. 2003. Quorum sensing in plant-pathogenic bacteria. Annu. Rev. Phytopathol. 41:12.1-12-28.
Teplitski, M., M. Gronquist, A. Eberhard, M. Gao, J. B. Robinson, B. G. Rolfe, and W. D. Bauer. 2003. Chemical identification of N-acyl homoserine lactone quorum sensing signals produced by Sinorhizobium meliloti strains in defined medium. Arch. Microbiol. 180:494-497.
Gao, M., M. Teplitski, J. B. Robinson and W. D. Bauer. 2003. Production of substances by Medicago truncatula that affect bacterial quorum sensing. Mol. Plant-Microbe Interact. 16:827-834.
Hirsch, A.M., W.D. Bauer, D.M. Bird, J. Cullimore, B. Tyler and J. I. Yoder. 2003. Molecular signals and receptors controlling rhizosphere interactions between plants and other organisms. Ecology 84:858-868
Bauer, W.D and U. Mathesius. 2004. Plant perception and disruption of bacterial quorum sensing. Curr. Opin. Plant Biol. 7:1-5.
Mathesius U, Pellerone FIA, Eberhard A, Nizamidin M, Behm CA, Bauer WD. 2004. Proteomic and behavioral responses of Caenorhabditis elegans to N-acyl-homoserine lactone quorum sensing signals of bacteria. Mol Ecol. In review
Teplitski, M., H. Chen, S. Rajamani, M. Gao, M. Merighi R.T. Sayre, J.B. Robinson, B.G. Rolfe and W.D. Bauer. 2004. Chlamydomonas secretes compounds that mimic bacterial signals and interfere with quorum sensing regulation in bacteria. Plant Physiology 134: 137-146
Last modified: 5/13/2008 |
