Presenter: Yi-Hsuan Chu
Advisor: Dr. Esther van der Knaap. Department of Horticulture and Crop Science
Proposal Type: Master Proposal
Regulatory Change in Tomato CLAVATA3 Gene by an Inversion Leads to fasciated Fruit
The diversity of tomato fruit shapes can be traced back to the selection outcomes from the domestication history. Tomato was originated in the Andean region in South America, followed by domestication in the Mesoamerican region and from there it spread to Europe and elsewhere. The spreading indirectly facilitates the expansion of fruit shape diversity from round to obovoid, ellipsoid, elongated, flat, and rectangular shapes. The fruit shape diversity can mostly be explained by OVATE, SUN, LC and FAS gens found in most tomato accessions throughout the world. Fruit shape gens, sun and ovate regulate elongated fruit shape; while other genes controlling fruit shapes, LC and FAS, can attribute to more locule number, which eventually leads to flat fruit shapes. LC, displaying weaker effects on increasing locule number, is located on the bottom of chromosome 2; while FAS presenting more pronounced effects on increasing locule number is located on the bottom of chromosome 11. However, the functions of these genes are still not well understood. In previous studies, LC is predicted to be WUSCHEL gene and fas mutation was proposed to be caused by ~294 kb inversion in the first intron of YABBY-like gene, which is speculated to disrupt the promoter region of CLV3-like gene. In this study, we are interested to investigate how CLV3-like gene leads to larger fruits with increased locule number, how LC gene interacts with FAS gene. Additionally, we would like to further explore any possible interaction between CLV3 and WUS-like genes and its impact on regulation of fruit shape.