Presenter: Qi Mu
Advisor: Dr. Esther van der Knaap, Department of Horticulture and Crop Science
Proposal Type: MS Proposal
Title of Presentation: Gene expression analysis and phenotypic evaluation of the tomato fruit weight 11.3 nearly isogenic lines.
Abstract: Tomato (Solanum lycopersicum) fruit size and shape have changed dramatically during the domestication process. The size and shape of produce is crucial to yield, quality, as well as growers, producers and consumer preferences. More than 30 QTLs related to tomato fruit size and shape have been identified, among which there are only two fruit weight genes—CNR (FW2.2) and SlKLUH (FW3.2) that have been cloned. Identifying the genes underlying fruit weight QTLs will not only clarify the molecular mechanism for fruit development, but also provide evolutionary insights of the domestication. The fw11.3 is an important QTL controlling tomato fruit weight. Fine mapping and association analysis has placed fw11.3 in a 15-kb region and one candidate gene, ORF2. Moreover, the enlarged fruit resulted from increasing cell size instead of increased cell number. We hypothesize that ORF2 is the underlying gene for fw11.3. The objectives of this research are 1) to verify whether the ORF2 gene is the underlying gene for fw11.3 by transgenic complementary experiments; 2) to analyze the expression pattern in fw11.3 NILs at 5 development fruit developmental stages and three tissue types using RNA-seq analyses; 3) to examine the cellular bases of fruit enlargement; 4) to collect phenotypic information of the fw11.3 NILs, such as ripening time, leaf size and plant structure.
November 13, 2013 - 11:30 AM
244 Kottman Hall (Columbus)
121 Fisher Auditorium (Wooster)