HCS Spring Seminar Series
Title: Genome editing of regulatory elements
Speaker: Dr. John Finer, Department of Horticulture and Crop Science
As the potential uses and benefits of genome editing continue to grow, the importance of this tool for both basic research and applied plant sciences has increased. A useful target for genome editing is the promoter region, which contains the regulatory elements that control expression of genes. The Finer Laboratory has been isolating and characterizing promoter regions by generating and analyzing promoter variants. For generation of these promoter variants, small changes are made to the promoter regulatory elements, which are placed upstream of a synthetic minimal promoter and reintroduced into plant tissue. Modified expression patterns in transgenic tissues are then monitored. More recently, genome editing approaches have been evaluated as an alternate approach for generation of promoter variants. With our increased knowledge of promoter functionality, and improvements in genome editing targeting efficiencies and base editing approaches, genome editing of the promoter region will likely be the method of choice for increasing native gene expression. Precise and controlled genome editing of the regulatory elements within the promoter region will result in minimal changes within the genome and allow both decreases and increases in native gene expression.
This presentation will provide some career perspectives on approaches to be considered for successful completion of research endeavors.