Dr. John Finer

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Dr. John Finer

Professor

finer.1@osu.edu

http://www.oardc.ohio-state.edu/plantranslab/default.htm

Office:

214 Williams Hall

Professional Information

Research Area(s):

Genetic Transformation and Transgene

Expression in Crop Plants

Biography:

AREA OF EXPERTISE

Research efforts in the laboratory have focused somatic embryogenesis, genetic transformation and promoter analysis in crop plants. Although the main emphasis in my laboratory is currently on soybean, we have additional experience with transformation of wheat, maize, sunflower, tomato, bentgrass, cotton, white pine, Chinese yam, Christmas cactus and Ohio buckeye. Embryogenic tissues are used for both basic studies on plant growth and development and as targets for transformation work. For our promoter work, we use image analysis to quantify expression of the gfp gene, under regulatory control of different promoters. Automated image collections is facilitated using a custom-designed robotics system, coupled with high-throughput image analysis.

Works

Journal Articles

Zhang N, McHale LK, Finer JJ, "A leader intron of a soybean elongation factor 1A (eEF1A) gene interacts with proximal promoter elements to regulate gene expression" PLoS ONE 11 11 (2016) 1-20-1-20

Hernandez-Garcia,Carlos,M; Finer,John,J, "Identification and validation of promoters and cis-acting regulatory elements." Plant Science 217-218 (2014) 109-119-109-119

Hernandez-Garcia CM, Finer JJ, "Identification and validation of promoters and cis-acting regulatory elements" Plant Science 217–218 (2014) 109–119-109–119

Pomeranz M, Finer J, Jang J-C, "Putative molecular mechanisms underlying tandem CCCH zinc finger protein mediated plant growth, stress, and gene expression responses." Plant Signaling & Behavior 5 6 (2011) 644-648-644-648

Pomeranz M, Zhang L, Finer J, Jang J-C, "Can AtTZF1 act as a transcriptional activator or repressor in plants?" Plant Signaling & Behavior 5 6 (2011) 644-648-644-648

Rushton DL, Tripathi P, Rabara R, Lin J, Ringler P, Boken AK, Langum TJ, Schmidt L, Boomsma DD, Emme N, Chen X, Finer JJ, Shen QJ, Rushton PJ, "WRKY transcription factors: key components in abscisic acid signaling" Plant Biotechnology Journal 2 10 (2011) 2-11-2-11

Benzle KA, Finer KR, Marty DM, McHale LK, Goodner BW, Taylor CG, Finer JJ, "Isolation and characterization of novel Agrobacterium strains for soybean and sunflower transformation" Plant Cell Tissue and Organ Culture

Zhang Z, Finer JJ, "Soybean actin, heat shock protein and ribosomal protein promoters direct tissue-specific transgene expression in transgenic soybean" In Vitro Cellular and Developmental Biology - Plant

Hernandez-Garcia, CM, Finer JJ, "Identification and validation of promoters and cis-acting regulatory elements" Plant Science

Chapters

"Transgenic Plant Production" In Plant Biotechnology and Genetics: Principles, Techniques and Applications. Edited by C. Neal Stewart. 2016.

Conferences

Hernandez-Garcia,Carlos,M; Nemes,Cheri; Jones,Michelle,L; Rushton,Paul,J; Finer,John,J, "Wound-induction of GmERF Promoters in Soybean" Society for In Vitro Biology 2012.

Hu,Bizhen; Scheerens,Joseph,C; Cardina,John; Finer,John,J, "Metabolite Production in Plant Cell Cultures of Burdock (Arctium sp.)" 2012.

De La Torre,Carola,M; Hernandez-Garcia,Carlos,M; Bouchard,Robert,A; Finer,John,J, "Dissection of the Strong Soybean Ubiquitin Promoter (Gmubi) Reveals Essential Regions Responsible for Regulating High Levels of Gene Expression" 2011.

Hernandez-Garcia,Carlos,M; Nemes,Cheri; De la Torre,Carola; Semsang,Nuananong; Jones,Michelle,L; Finer,John,J, "GmERF Promoters Give High Wound-inducible Expression in Transgenic Soybean" Society for In Vitro Biology 2011.

Zhang,Zhifen; Finer,John,J, "Development of a Transformation and Regeneration System for Sunflower (Helianthus annus)" 2011.

Waterland, NL, Finer, JJ, Jones, ML, "Abscisic Acid Applications Decrease Stomatal Conductance and Delay Wilting in Drought-stressed Chrysanthemums" 106th Annual Conference of the American-Society-for-Horticultural-Science 2010.

Bouchard,R,A; Chiera,J,M; Finer,J,J, "Isolation and characterization of a soybean (Glycine max) polyubiquitin (Gmubi) promoter." 2007.

Chiera,J,M; Finer,J,J, "Evaluation of an automated image analysis system for factors which stabilize gene expression." Society for In Vitro Biology 2006.

Chiera,J,M; Streeter,J,G; Nemes,C,A; Finer,J,J, "The effect of Myo-inositol concentration on pinitol levels in Inositol methyl transferase (imt)-containing transgenic embryonic soybean" Society for In Vitro Biology 2005.

Patents

Zhang Z and Finer JJ. Low inoculum, long coculture Agrobacterium mediated transformation of plants. Filed Jan 2016

Finer JJ, Benzle KA, Finer KR, Taylor CG. Agrobacterium Strains for Plant Transformation and Related Materials and Methods. Filed May 2015

Finer JJ, Benzle KA, Finer KR, Taylor CG. Agrobacterium Strains for Plant Transformation and Related Materials and Methods. Filed May 2014

John J. Finer and Robert A. Bouchard. Highly Active Soybean Promoters and Uses Thereof. Filed Feb 2013

John J Finer; Robert A Bouchard. Highly Active Soybean Promoters and Uses Thereof. 8395021 Filed May 2008 and Issued May 2013.

Finer,John,J; Trick,Harold,N. Method for transforming plant tissue by sonication. 5693512 Filed Mar 1996 and Issued Dec 1997.

Presentations

"Plant GMOs." In College of Wooster Undergraduate seminar. 2016.

"Development and use of an automated image capture system for semi-continuous monitoring of promoter-mediated gene expression." In Society for In Vitro Biology. 2016.

"Isolation and characterization of soybean promoters and their contributing cis-elements." In Invited seminar. 2016.

"Development of low inoculm, long co-culture method for sunflower transformation." In National Sclerotinia Initiative. 2016.

"Transgene expression analysis in soybean." In Department Seminar. 2015.

"A novel Agrobacterium strain for soybean transformation." In Bayer CropScience. 2015.

"The soybean tissue culture and genetic engineering center." In USB Nematode Project Meeting. 2015.

"Soybean tissue culture." In Soybean Doubled Haploids Workshop. 2015.

"Use of a Transformation System in Sunflower for Sclerotinia Resistance Studies." In National Sclerotinia Initiative. 2015.

"Isolation and evaluation of novel Agrobacterium strains for transformation of soybean and sunflower." In Crown Gall Meetings. 2014.

"Improvements in sunflower transformation using cotyledon explants from mature seeds." In Crown Gall Meeting. 2014.

"Identification and validation of regulatory elements from soybean promoters." In Purdue University Department of Agronomy Seminar Series. 2014.

"Soybean Promoteromics." In The Ohio State University Department of Plant Pathology Seminar Speaker. 2014.

"Isolation and Characterization of Soybean Urease Promoters." In Soy2014. 2014.

"Publication Ethics, Plagiarism." In Society for In Vitro Biology Annual Meeting. 2014.

"Development of Sunflower Transformation through the Use of Cotyledon Explants from Mature Seeds." In Society for In Vitro Biology Annual Meeting. 2014.

"Transgene expression analysis in soybean." In Ohio State University Department of Horticulture and Crop Science Seminar Series. 2014.

"Novel Pathogen Responsive Promoters and Cis-regulatory Elements in Soybean." In 2014 Plant and Animal Genome Meetings. 2014.

"Use of a Transformation System in Sunflower for Sclerotinia Resistance Studies." In National Sclerotinia Initiative annual meeting. 2014.

"Soybean promoter isolation and characterization." In Kansas State University. 2013.

"Use of a Transformation System in Sunflower for Sclerotinia Resistance Studies." In National Sclerotinia Initiative annual meeting. 2013.

"Identification of regulatory regions for control of transgene expression." In International Association for Plant Biotechnology - Canadian Division. 2012.

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Dr. John Finer

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